| 产品编号 | bsm-60152R |
| 英文名称 | Rabbit Anti-Mono-Methyl-Histone H4 (Lys12) antibody |
| 中文名称 | |
| 产品类型 | 甲基化抗体 重组兔单抗 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | B11E4 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1:50-1:100,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 组蛋白翻译后修饰，也称作“组蛋白密码”，通过调控染色质结构发挥作用，是表观遗传学的重要研究分支。 组蛋白上的翻译后修饰包括乙酰化，甲基化，磷酸化和近年发现的一些新型酰化修饰。这些组蛋白修饰直接影响染色质和转录因子或其他表观调控子的结合，改变基因组的稳定性和基因转录等。组蛋白甲基化通常发生在核心组蛋白的赖氨酸和精氨酸侧链氨基上。组蛋白甲基化既可以促进也可以抑制基因转录，这取决于组蛋白甲基化是发生在赖氨酸还是发生在精氨酸上以及甲基化基团的个数（赖氨酸可以发生一、二、三甲基化，精氨酸可发生单、对称与非对称二甲基化）。组蛋白赖氨酸甲基化通常发生在组蛋白H3第4，9，27，36，79和组蛋白H4的第5, 8, 12, 20位赖氨酸上；精氨酸甲基化通常发生在组蛋白H3的第2，8，17，26和组蛋白H4第4位精氨酸上。蛋白甲基化酶（HMT）和组蛋白去甲基化酶（HDM）是甲基化修饰主要的调控因子。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1. HeLa, 2. NIH-3T3, 3. BRL, 4. Mouse heart，5. Rat heart, 6. Mouse kidney, 7. Rat kidney, 8. Recombinant Histone H4
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human prostate hyperplasis
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60152R
Tissue: Mouse liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60152R
Tissue: Rat stomach
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60152R
Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60152R
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60152R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
