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Rabbit Anti-Histone H3 (Tri Methyl K36)  antibody (bsm-60113R)  
~~~促销代码KT202411~~~
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说明书: 50ul  100ul  
50ul/1580.00元
100ul/2500.00元
大包装/询价

产品编号 bsm-60113R
英文名称 Rabbit Anti-Histone H3 (Tri Methyl K36)  antibody
中文名称 三甲基化组蛋白H3抗体
别    名 H3K36me3; Tri-Methyl-Histone H3 (Lys36); Histone H3(Tri Methyl Lys36); Tri methyl Histone H3(Lys36); Tri methyl Histone H3(K36); H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD;  
产品类型 甲基化抗体 重组兔单抗 
抗体来源 Rabbit
克隆类型 Recombinant
克 隆 号 B3C9
交叉反应 Human,Mouse,Rat
产品应用 WB=1:500-1:2000,IHC-P=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of Tri Methyl K36 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.

SWISS:
P68431

Gene ID:
8350

组蛋白翻译后修饰(PTMs)是表观遗传学调控染色质结构的关键机制,被称为“组蛋白密码”。组蛋白上的翻译后修饰包括乙酰化,甲基化,磷酸化和近年发现的一些新型酰化修饰。这些组蛋白修饰直接影响染色质和转录因子或其他表观调控子的结合,改变基因组的稳定性和基因转录等。组蛋白甲基化通常发生在核心组蛋白的赖氨酸和精氨酸残基。组蛋白甲基化既可以促进也可以抑制基因转录,这取决于组蛋白甲基化是发生在赖氨酸还是发生在精氨酸上以及甲基化基团的个数(赖氨酸可以发生一、二、三甲基化,精氨酸可发生单、对称与非对称二甲化)。组蛋白赖氨酸甲基化通常发生在组蛋白H3第4,9,27,36,79和组蛋白H4的第20位赖氨酸上;精氨酸甲基化通常发生在组蛋白H3的第2,8,17,26和组蛋白H4第3位精氨酸上。蛋白甲基化酶(HMT)和组蛋白去甲基化酶(HDM)是甲基化修饰主要的调控因子。
产品图片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1. MCF-7, 2. NIH-3T3, 3. HepG2, 4. Rat kidney, 5. Mouse kidney, 6. Recombinant Histone H3 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa
Tissue: Human colon cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60113R
Tissue: Mouse stomach Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60113R
Tissue: Rat kidney Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60113R
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