产品编号 | bs-41390R |
英文名称 | Rabbit Anti-PPV VP2 antibody |
中文名称 | 猪细小病毒VP2蛋白抗体 |
别 名 | PPV-VP2; Porcine parvovirus VP2; PPV Isoform VP2(P18546-2); Capsid protein VP1; CAPSD_PAVPN. |
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Specific References (1) | bs-41390R has been referenced in 1 publications.
[IF=6.286] Qianqian Xie. et al. Inhibition of Transcription of VP2 by Mutations in the DNA Binding Domains of Mink Enteritis Virus NS1 protein. VIRUS RES. 2022 Oct;:198972 IF ; Cat.
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研究领域 | 细菌及病毒 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Pig,PPV |
产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 64/81 kDa |
检测分子量 | |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Recombinant PPV VP2 protein : 1-579/579 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Porcine Parvovirus Infection (PPV) is the most common and important cause of infectious infertility. Porcine parvovirus is a fairly tough virus that multiplies normally in the intestine of the pig without causing clinical signs. It is world-wide in its distribution. If you test for it in your pig herd it is almost certain it will be present unless your herd is less than 100 sows when it might have died out. It is therefore an infection you have to live with and manage. Whereas most viruses do not survive outside the host for any great period of time PPV is unusual in that it can persist outside the pig for many months and it is resistant to most disinfectants. This perhaps explains why it is so widespread and so difficult to remove from the pig environment. |
产品图片 |
Sample:
Lane 1: Recombinant PPV-VP2 protein, N-His
Primary: Anti-PPV VP2 (bs-41390R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64/81 kDa
Observed band size: 70 kDa
Measured by its binding ability in a indirect ELISA. Immobilized recombinant PPV-VP2 protein, His Tag (Cat. bs-41390P) at 2 μg/mL (100 μL/well) can bind rabbit Anti-PPV-VP2 polyclonal antibody, the EC50 is 181.0 ng/mL.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |