| 产品编号 | bs-42013R |
| 英文名称 | Rabbit Anti-CD68 antibody |
| 中文名称 | CD68抗体 |
| 别 名 | CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68 (microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP |
| 研究领域 | 免疫学 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat) |
| 产品应用 | Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35kDa |
| 检测分子量 | 110-140 |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse CD68 protein: 119-298/326 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008] SWISS: P31996 Gene ID: 12514 Database links: Entrez Gene: 968 Human Entrez Gene: 12514 Mouse Omim: 153634 Human SwissProt: P34810 Human SwissProt: P31996 Mouse Unigene: 647419 Human Unigene: 15819 Mouse |
| 产品图片 |
Blank control(black line):Raw264.7
Primary Antibody (green line): Rabbit Anti-CD68 antibody (bs-42013R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control(orange line)：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(black line):Raw264.7
Primary Antibody (green line): Rabbit Anti-CD68 antibody (bs-42013R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control(orange line)：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Raw264.7.
Primary Antibody (green line): Mouse Anti-CD68 antibody (bs-42013R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Raw264.7.
Primary Antibody (green line): Mouse Anti-CD68 antibody (bs-42013R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag (Cat. bs-42013P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse CD68 Antibody, the EC50 is 142.5 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD68 protein, His Tag (Cat. bs-42013P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Mouse CD68 Antibody, the EC50 is 175.4 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
