| 产品编号 | bs-41347R |
| 英文名称 | Rabbit Anti-Adenosine A3 Receptor antibody |
| 中文名称 | 腺苷受体A3抗体 |
| 别 名 | adenosine A3 receptor; A3AR; AD 026; AD026; ADORA 3; ADORA3; bA552M11.5; RP11 552M11.7; TGPCR1; adenosine receptor A3 isoform 1; adenosine receptor A3 isoform 2; AA3R_HUMAN. |
| 研究领域 | 心血管 神经生物学 细胞膜受体 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Adenosine receptor A3 protein: 270-318/347 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. SWISS: P0DMS8 Gene ID: 140 Database links: Entrez Gene: 140 Human Omim: 600445 Human SwissProt: P0DMS8 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (bs-41347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (bs-41347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Measured by its binding ability in a indirect ELISA. Immobilized Human Adenosine A3 protein, His Tag (Cat. bs-41347P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti- Human Adenosine A3 Antibody, the EC50 is 116.5 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
