| 产品编号 | D50453 |
| 英文名称 | Okadaic acid |
| 中文名称 | 冈田酸 |
| 别 名 | OA; Okadaic A; 35-Demethyldinophysistoxin 1 solution; 35-Demethyl-DTX 1 solution; NSC 677083; PP1&PP2A inhibitor; PP1 and PP2A inhibitor; Okadaic acid potassium salt from Prorocentrum; 冈田(软海绵)酸; 软海绵酸; 黑海棉酸; 冈田酸/软海绵酸; 冈田酸 钾盐 来源于凹形原甲藻;PP1和PP2A抑制剂 |
| 克 隆 号 | |
| CAS | 155751-72-7 |
| 理论分子量 | kDa |
| 检测分子量 | |
| 保存条件 | Store at -20℃ stable for 24 months. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
基本信息： CAS：155751-72-7 分子式：C44H67KO13 分子量：843.09
MDL：MFCD00214356 溶解性：溶于乙醇（5 ug/50 ul = 124.2 μM） 产品简介： 冈田酸 (Okadaic acid) 是一种海洋毒素，是蛋白质磷酸酶 (PP) 的抑制剂，包括 PP1 (IC50=15-50 nM)、PP2A (IC50=0.1-0.3 nM)、PP3 (IC50=3.7-4 nM)、PP4 (IC50=0.1 nM) 和 PP5 (IC50=3.5 nM)，其中对 PP2A 的亲和力最显著，但不抑制 PP2C。Okadaic acid 通过抑制 PP 增加了多种蛋白的磷酸化，并具有肿瘤启动子的作用。Okadaic acid 诱导 tau 蛋白磷酸化。 作用靶点：Phosphatase; Apoptosis; 作用通路：Metabolic Enzyme/Protease; Apoptosis 体外研究： Okadaic acid (0-100 nM; 24 h or 48 h) inhibits the proliferation of AGS, MNK-45, Caco 2 cells[3]. Okadaic acid (10 nM; 8 hours) increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation Assay[3] 体内研究： Okadaic acid (100 μM; injected unilaterally to the lateral amygdala) induces Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection[5] REFERENCES： [1]. Kleppe R, et al. Cell Death Inducing Microbial Protein Phosphatase Inhibitors--Mechanisms of Action. Mar Drugs. 2015 Oct 22;13(10):6505-20. [2]. Valdiglesias V, et al. Okadaic acid: more than a diarrheic toxin. Mar Drugs. 2013 Oct 31;11(11):4328-49. [3]. del Campo M, et al. Okadaic acid toxin at sublethal dose produced cell proliferation in gastric and colon epithelial cell lines. Mar Drugs. 2013;11(12):4751-4760. [4]. Cho MH, et al. Increased phosphorylation of dynamin-related protein 1 and mitochondrial fission in okadaic acid-treated neurons. Brain Res. 2012 May 15;1454:100-10. [5]. Baker S, et al. A local insult of okadaic acid in wild-type mice induces tau phosphorylation and protein aggregation in anatomically distinct brain regions. Acta Neuropathol Commun. 2016;4:32. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
