| 产品编号 | bs-43033R |
| 英文名称 | TIGIT Rabbit pAb |
| 中文名称 | TIGIT抗体 |
| 别 名 | VSIG9; VSTM3; WUCAM; TIGIT_HUMAN; TIGIT; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3; |
| 研究领域 | 肿瘤 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 26 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human TIGIT protein: 22-141/244 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009] SWISS: Q495A1 Gene ID: 201633 Database links:
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| 产品图片 |
Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Rat Lymph node tissue lysates
Primary: Anti-TIGIT (bs-43033R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 35 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human TIGIT protein, HisTag (Cat. bs-43033P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human TIGIT Antibody, the EC50 is 152.5 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human TIGIT protein, His Tag (Cat. bs-43033P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human TIGIT Antibody, the EC50 is 1070 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
