产品编号 | bs-25244R |
英文名称 | Rabbit Anti-Endostatin antibody |
中文名称 | 内皮抑素/内皮他丁抗体 |
别 名 | Collagen XV alpha 1; alpha 1 collagen type 18(XVIII)(COL18A1); alpha 1 type XVIII collagen; COL18A1; Collagen alpha 1(XVIII) chain; Collagen type XVIII alpha 1; FLJ27325; KNO; MGC74745; COL18A1; COIA1_HUMAN; Antiangiogenic agent; COIA1_HUMAN; COL15A1; COL18A1; Collagen alpha 1(XV) chain; Collagen alpha-1(XV) chain; Collagen type XV proteoglycan; Collagen type XVIII alpha 1; Collagen XV, alpha 1 polypeptide; Collagen, type XV, alpha 1; Endostatin; Endostatin XV; FLJ34914; FLJ38566; KNO1; KS; Multi functional protein MFP; OTTHUMP00000021782; OTTHUMP00000115472; OTTHUMP00000115473. |
研究领域 | 肿瘤 免疫学 生长因子和激素 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Dog) |
产品应用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 190 kDa |
检测分子量 | |
细胞定位 | 细胞外基质 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Endostatin |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Two transcript variants encoding different isoforms have been found for this gene. SWISS: P39060 Gene ID: 80781 |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Endostatin) Polyclonal Antibody, Unconjugated (bs-25244R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |