| 产品编号 | bs-25232R |
| 英文名称 | Rabbit Anti-FCGR1A/CD64 antibody |
| 中文名称 | 免疫球蛋白G Fc段受体I |
| 别 名 | High affinity immunoglobulin gamma Fc receptor I; CD 64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ia receptor; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc fragment of IgG, high affinity Ib, receptor (CD64); Fc fragment of IgG, high affinity Ib, receptor for; Fc fragment of IgG, high affinity Ic, receptor (CD64); Fc fragment of IgG, high affinity Ic, receptor for; Fc gamma receptor; Fc gamma receptor I A1; Fc gamma receptor I; Fc gamma receptor I B2; Fc gamma RI; Fc of IgG high affinity Ia receptor; Fc-gamma RIC; FCG 1; FCG1; FcgammaRI; FCGR 1; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcRI; FCRIC; IGFR 1; IGFR1; IGFRB; IGFRC; IgG Fc receptor I; IgG Fc receptor IC; Immunoglobulin G Fc receptor I; Immunoglobulin G Fc receptor IB; Immunoglobulin G Fc receptor IC. |
| 研究领域 | 细胞生物 免疫学 细胞膜受体 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse FCGR1A/CD64 : 131-230/404 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008] Subunit: Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G. Tissue Specificity: Monocyte/macrophage specific. Similarity: Belongs to the immunoglobulin superfamily. FCGR1 family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P26151 Gene ID: 14129 Database links: Entrez Gene: 2209 Human Omim: 146760 Human SwissProt: P12314 Human Unigene: 77424 Human CD64是一种糖蛋白，主要定位于单核细胞和巨噬细胞,参与免疫应答反应。 |
| 产品图片 |
Sample:
Lane 1: Mouse Lung tissue lysates
Primary: Anti-FCGR1A/CD64 (bs-25232R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 62 kDa
Blank control（black line）:Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD64 antibody (bs-25232R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat Anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
