| 产品编号 | bsm-60359M |
| 英文名称 | Annexin IV Recombinant Mouse mAb |
| 中文名称 | 膜粘连蛋白A4重组鼠单抗 |
| 别 名 | 35 beta calcimedin; Carbohydrate binding protein P33/P41; DKFZp686H02120; Endonexin I; Lipocortin IV; P32.5; Annexin A4; Annexin IV placental anticoagulant protein II; ANX 4; ANX A4; ANX4; ANXA 4; LipocortinIV; P33/41; Placental anticoagulant protein II antibody PP4X; Proliferation inducing protein 28; ANXA4_HUMAN. |
| 研究领域 | 心血管 |
| 抗体来源 | Mouse |
| 克隆类型 | Recombinant |
| 克 隆 号 | D4C7 |
| 交叉反应 | Human |
| 产品应用 | WB=1:200-1000,IHC-P=1:20-100,IHC-F=1:400-800,IF=1:20-50,ICC/IF=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Annexin IV |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin A4 (ANXA4) belongs to the annexin family of calcium dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane related events along exocytotic and endocytotic pathways. ANXA4 has 45 to 59% identity with other members of its family and shares a similar size and exon intron organization. Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells. Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P09525 Gene ID: 307 Database links: Entrez Gene: 307 Human Entrez Gene: 11746 Mouse Omim: 106491 Human SwissProt: P09525 Human SwissProt: P97429 Mouse Unigene: 422986 Human Unigene: 259702 Mouse Unigene: 19270 Rat |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition:4°C overnight
Lysate: HeLa, HepG2, SW480, SH-SY5Y
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 32 kDa
Observed MW: 32 kDa
Tissue: Human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60359M
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60359M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
