产品编号 | bsm-60231R |
英文名称 | Rabbit Anti-CD7 antibody |
中文名称 | CD7重组兔单抗 |
别 名 | CD7 antigen (p41); CD7 molecule; CD7_HUMAN; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; CD 7; CD7 antigen; CD7 antigen p41; CD7 molecule; GP 40; GP40; LEU 9; LEU9; p41; p41 protein; T cell antigen CD7; T cell antigen CD7 precursor; T cell leukemia antigen; Tp 40; TP 41; Tp40; TP41. |
研究领域 | 细胞生物 免疫学 t-淋巴细胞 |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | B2D9 |
交叉反应 | Human |
产品应用 | IHC-P=1:200-1000,IHC-F=1:400-800,Flow-Cyt=1:50,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 24kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CD7 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]
Subunit: Interacts with SECTM1. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like (immunoglobulin-like) domain. SWISS: P09564 Gene ID: 924 Database links: Entrez Gene: 924 Human Entrez Gene: 12516 Mouse Omim: 186820 Human SwissProt: P09564 Human SwissProt: P50283 Mouse Unigene: 36972 Human Unigene: 4100 Mouse CD7是一种40kDa的糖蛋白,为Ig超级基因家族的成员,在绝大多数成熟与不成熟T细胞和T细胞白血病中表达,但也表达于NK细胞、小部分正常B细胞和恶性B细胞。如T细胞其它标记物抗体无效,可用CD7来鉴别原始T细胞肿瘤如淋巴母细胞白血病。 |
产品图片 |
Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60231R
Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60231R
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60231R
Tissue: Human spleen
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60231R
Cell line: Jurkat
Fixation: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:50
Secondary Ab: Goat Anti-Rabbit IgG
Unlabelled control: The cell without incubation
with primary antibody and secondary antibody
(Black line).
Isotype control: Rabbit monoclonal IgG (Blue
line).
Comment: Line red is the positive signal for
bsm-60231R
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |