| 产品编号 | bsm-60352M |
| 英文名称 | CYP11A1 Recombinant Mouse mAb |
| 中文名称 | 细胞色素P450 11A1重组鼠单抗 |
| 别 名 | Cholesterol 20 22 desmolase; Cholesterol desmolase; Cholesterol monooxygenase(side chain cleaving); Cholesterol side chain cleavage enzyme; Cholesterol side chain cleavage enzyme mitochondrial; Cholesterol side-chain cleavage enzyme; CP11A_HUMAN; CYP11A1; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450 11A1 mitochondrial; Cytochrome P450 family 11 subfamily A polypeptide 1; Cytochrome P450 subfamily XIA; Cytochrome P450(scc); Cytochrome P450C11A1; mitochondrial; P450SCC; Steroid 20 22 lyase. |
| 研究领域 | 肿瘤 心血管 免疫学 信号转导 |
| 抗体来源 | Mouse |
| 克隆类型 | Recombinant |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-1000,IHC-P=1:20-100,IHC-F=1:400-800,IF=1:20-100,ICC/IF=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53/57 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP11A1 |
| 亚 型 | IgG1, Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] SWISS: P05108 Gene ID: 1583 Database links: Entrez Gene: 1583 Human Entrez Gene: 13070 Mouse SwissProt: P05108 Human SwissProt: Q9QZ82 Mouse |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: JAR, HepG2
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 52 kDa
Observed MW: 52 kDa
Tissue: Human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab:SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60352M
Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60352M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
