| 产品编号 | bsm-30074M |
| 英文名称 | Mouse Anti-human CD14 antibody |
| 中文名称 | 小鼠抗人CD14单克隆抗体 |
| 别 名 | Monocyte Differentiation Antigen 14; CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_HUMAN; Myeloid cell-specific leucine-rich glycoprotein. |
| 研究领域 | 细胞生物 免疫学 干细胞 细胞表面分子 细胞类型标志物 淋巴细胞 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 14F4 |
| 交叉反应 | Human |
| 产品应用 | Flow-Cyt=1ul/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35/40kDa |
| 检测分子量 | 53-55 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | Mouse IgG1, k |
| 纯化方法 | Affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH=7.4) |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010] Function: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. Subunit: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Similarity: Contains 11 LRR (leucine-rich) repeats. SWISS: P08571 Gene ID: 929 Database links: Entrez Gene: 929 Human Entrez Gene: 12475 Mouse Omim: 158120 Human SwissProt: P08571 Human Unigene: 163867 Human CD14又称内毒素受体，属细胞表面糖蛋白家族成员之一。在机体免疫、防御系统引起的一系列反应中介导细胞识别LPS并引起细胞酪氨酸磷酸化、核因子NF-κB转位、触发细胞因子释放和氧自由基产生。CD14在细胞因子产生的调控方面起重要作用,因而对于CD14结构、功能及其临床意义的研究日益受到重视。 |
| 产品图片 |
Blank control:whole blood.
Primary Antibody (green line): Mouse Anti-CD14 antibody (bsm-30074M)
Dilution: 1ul/Test;
Secondary Antibody : Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Protocol
Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
