| 产品编号 | D10692 |
| 英文名称 | Recombinant Cholera Toxin B subunit |
| 中文名称 | 重组霍乱毒素B亚基 |
| 别 名 | Cholera Toxin B subunit; B subunit Cholera Toxin; Beta subunit Cholera Toxin; CTxB; Choleragenoid; CTB; CHTB; 霍乱毒素亚型B(CTB); 霍乱毒素B亚基; 霍乱毒素B亚单位; |
| 克 隆 号 | |
| CAS | 24730-31-2 |
| 理论分子量 | kDa |
| 检测分子量 | |
| 保存条件 | Store at 2-8℃. DO NOT FREEZE. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
基本信息： CAS：131096-89-4 分子量：11 kDa 种属：Vibrio cholerae 来源：E. coli. 内毒素：Less than 0.1EU/µg of rCTB as determined by LAL method. 纯度：>98% by SDS-PAGE and HPLC analyses. 外观：Sterile colorless liquid. 缓冲体系：Supplied as a 0.2µM filtered solution in 5mM PB, pH7.0, 75mM NaCl, with 50% glycerol. (Sodium azide free.) 描述：霍乱毒素B亚基 (Cholera Toxin B subunit）属于毒素的AB5-亚单位家族。天然六聚体蛋白的分子量约为85 kDa，包含两个亚单位。它由负责ADP核糖基化活性的单个a亚单位（~27.2 kDa）和五个B亚单位（~11.6 kDa）组成，排列为五聚环，具有明显的5倍对称性，与细胞表面受体结合和酶组分的后续内化（跨膜转运）有关。 背景资料： Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. CTX is responsible for the massive, watery diarrhea characteristic of cholera infection. The cholera toxin is an oligomeric complex made up of six protein subunits: a single copy of the A subunit (part A, enzymatic), and five copies of the B subunit (part B, receptor binding), denoted as AB5. Subunit B binds while subunit A activates the G protein which activates adenylate cyclase. The five B subunits - each weighing 11 kDa, form a five-membered ring. The A subunit which is 28 kDa, has two important segments. The A1 portion of the chain (CTA1) is a globular enzyme payload that ADP-ribosylates G proteins, while the A2 chain (CTA2) forms an extended alpha helix which sits snugly in the central pore of the B subunit ring. This structure is similar in shape, mechanism, and sequence to the heat-labile enterotoxin secreted by some strains of the Escherichia coli bacterium. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
