| 产品编号 | bs-22241R |
| 英文名称 | Rabbit Anti-GDF15 antibody |
| 中文名称 | 生长分化因子15/巨嗜细胞抑制因子1抗体 |
| 别 名 | GDF 15; GDF-15; GDF15_HUMAN; Growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC 1; Mic-1; MIC1; NAG 1; NAG-1; NAG1; NRG 1; NRG-1; NRG1; NSAID (nonsteroidal anti inflammatory drug) activated protein 1; NSAID; NSAID regulated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; Placental bone morphogenic protein; Placental TGF beta; Placental TGF-beta; Prostate differentiation factor; PTGF beta; PTGFB. |
| 研究领域 | 心血管 细胞生物 信号转导 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12.5/34kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDF15 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Growth differentiation factor 15 (GDF15) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. It has been implicated in a variety of functions directly related to tumorigenicity including antiproliferative and pro-apoptotic effects. BMP proteins are secreted growth factors that are characterized by seven conserved cysteine residues. In general, they are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF15 is an important downstream mediator of DNA damage signaling and a transcriptional target of p53. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. Similarity: elongs to the TGF-beta family. SWISS: Q99988 Gene ID: 9518 Database links: Entrez Gene: 9518 Human Entrez Gene: 23886 Mouse Omim: 605312 Human SwissProt: Q99988 Human SwissProt: Q9Z0J7 Mouse Unigene: 616962 Human Unigene: 31325 Mouse Unigene: 44228 Rat 生长分化因子15（GDF15）是骨形态发生蛋白（BMP）和转化生长因子-β超家族成员。GDF-15又叫巨噬细胞抑制因子（macrophage inhibiting cytokine，MIC）-1、胎盘转化生长因子 （placental transformation growth factor，PTGF）-β、前列腺衍生因子（prostate derived factor，PDF）和胎盘骨形态发生蛋白（placental bone morphogeneticprotein，PLAB）。 有学者认为：GDF-15在肿瘤发展的不同阶段发挥着不同的生物学作用；随着肿瘤的发生发展，GDF-15可能表达不同形式的蛋白，在肿瘤发生的早期阶段可能发挥抑瘤作用，而在肿瘤发展的后期阶段发挥促瘤作用。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with GDF15 polyclonal antibody, unconjugated (bs-22241R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
