| 产品编号 | bsm-60020R |
| 英文名称 | ERBB2/HER2 Recombinant Rabbit mAb |
| 中文名称 | ERBB2/HER2重组兔单抗 |
| 别 名 | erbB-2 isoform 2; HER2 receptor; Erbb2 protein; CerbB2; c erb B2; C erbB 2; C-erbB2; CD340; Erb B2; erbb2; HER 2; Her-2; HER 2/neu; Her2/neu; Herstatin; MLN 19; MLN19; NEU; NEU Proto Oncogene; Neuro Glioblastoma Derived Oncogene Homolog; NGL; p185 ErbB2; p185erbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Receptor tyrosine protein kinase erbB 2; TKR1; Tyrosine kinase type cell surface receptor HER2; v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog(avian); ERBB2_HUMAN; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 生长因子和激素 激酶和磷酸酶 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 185 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ERBB2/HER2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]. Function: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. Subunit: Heterodimer with each of ,the other ERBB receptors. The constitutively activated oncogenic variant forms a homodimer. Interacts with PRKCABP and PLXNB1. It is a type I membrane protein. Ligand-binding increases phosphorylation on tyrosine residues Belongs to the Tyr protein kinase family. EGF receptor subfamily. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus. Isoform 2: Cytoplasm. Nucleus. Isoform 3: Cytoplasm. Nucleus. Tissue Specificity: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. Post-translational modifications: Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. DISEASE: Defects in ERBB2 are a cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. A cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain. SWISS: P04626 Gene ID: 2064 Database links: Entrez Gene: 2064 Human Entrez Gene: 13866 Mouse Omim: 164870 Human SwissProt: P04626 Human SwissProt: P70424 Mouse Unigene: 446352 Human Unigene: 290822 Mouse Unigene: 93966 Rat 细胞膜受体（Membrane Receptors) c-erbB-2癌基因，在很多癌组织中呈C-erbB-2过度表达。cerbB尤其临床常用于乳腺癌术后C-erbB-2蛋白、雌激素受体（ER）和孕激素受体（PR）表达情况，旨在进一步探讨癌基因蛋白，细胞增殖活性和激素受体与乳腺癌组织学分级及术后生存期的关系，为乳腺癌的病理诊断、预后判断及治疗提供依据。 |
| 产品图片 |
Tissue:Human breast cancer
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour a
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
