| 产品编号 | bsm-51474M |
| 英文名称 | LAMP2 Mouse mAb |
| 中文名称 | 溶酶体相关膜蛋白2（CD107B）单克隆抗体 |
| 别 名 | CD107 antigen-like family member B; CD107b; CD107b antigen; Igp110; Igp2; LAMP 2; LAMP 2C; LAMP B; LAMP-2; LAMP2_RAT; LAMPB; LGP110; Lysosomal associated membrane protein 2C; Lysosome associated membrane glycoprotein 2 precursor; Lysosome associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2; MAC3. |
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Specific References (1) | bsm-51474M has been referenced in 1 publications.
[IF=7.666] Kenji Ono. et al. Calmodulin as a Key Regulator of Exosomal Signal Peptides. CELLS-BASEL. 2023 Jan;12(1):158 ICC ; Human.
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| 研究领域 | 肿瘤 心血管 细胞生物 神经生物学 信号转导 新陈代谢 线粒体 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | L3G4 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:10-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human LAMP2 |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by the LAMP2 gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands and is thought to play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of the gene produces two known products, LAMP2a and LAMP2b. Isoform LAMP2a is highly expressed in placenta, lung and liver and has low expression in brain and skeletal muscle. The isoform detected by this antibody, LAMP2b, is highly expressed in skeletal muscle less so in brain, placental, lung, kidney and pancreas and has very low expression in liver. Function: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Post-translational modifications: O- and N-glycosylated; some of the N-glycans attached to Lamp-2 are polylactosaminoglycans. Similarity: Belongs to the LAMP family. SWISS: P13473 Gene ID: 3920 Database links: Entrez Gene: 3920 Human SwissProt: P13473 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with LAMP2 monoclonal antibody, unconjugated (bsm-51474M) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
