| 产品介绍 |
Superoxide dismutase plays an important role in the balance of oxidation and anti-oxidation in vivo and can eliminate the superoxide anions in vivo to avoid cell damage by the superoxide anions.Super oxide anions generated as the byproduct of Xanthine Oxidase catalyzed Xanthine oxidation and the anion can oxidize hydroxylamine to nitrite which appears to be Amaranth purple in the presence of chromogenic agent and thus the absorbance at certain wavelength can be detected by spectrophotometer. The presence of SOD in the system specifically inhibits the oxidization caused by super oxide anions and because of it, fewer nitrite anions are generated. This would lower the absorbance of the sample tube compared to the reference tube without SOD and the SOD activity can be calculated with the formula given.
There are two types of SODs in higher animal cells: CuZn-SOD and Mn-SOD with total SOD activity equals to the sum of the activities of both type. Pretreatment should be done to remove the activity of Mn-SOD in sample while retain its original CuZn-SOD activity.
This assay kit is designed to measure the activity of superoxide dismutase based on the xanthine oxidase method and is suitable for the samples like serum/plasma, cerebrospinal fluid, pleural effusion, peritoneal fluid, renal dialysis fluid, urine, red blood cells, white blood cells, platelets, cultured myocardium cells, cultured neoplasm, and other cells or subcellular components (mitochondria or microsome etc.). Also, researchers can use this kit for the measurement of samples such as micro-organisms, drugs, food, beverages and cosmetics.
超氧化物歧化酶对机体的氧化与抗氧化平衡起着至关重要的作用。此酶能清除超氧阴离子自由基，保护细胞免受损伤，本试剂盒采用黄嘌呤氧化酶法(羟胺法)测定SOD活力。可测血清(浆)、脑脊液、胸水、腹水、肾透析液、尿液、精液、红细胞、白细胞、血小板、心肌培养细胞、肿瘤培养细胞、各种动植物组织细胞、及亚细胞水平(线粒体、微粒体)中的SOD活力，并可检测微生物、药物、食品、饮料、化妆品中的SOD活力。既可测总SOD又可测锰-SOD及铜锌-SOD。
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