Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Testis tissue lysates Primary: Anti-H2-Q10 (bs-49099R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (bs-49099R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (bs-49099R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control:Raw264.7. Primary Antibody (green line): Rabbit Anti-H2-Q10 antibody (bs-49099R) Dilution: 1ug/Test; Secondary Antibody (green line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Negative control（white blue line）：PBS Isotype control（orange line）：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:Raw264.7. Primary Antibody (green line): Rabbit Anti- H2-Q10 antibody (bs-49099R) Dilution: 1ug/Test; Secondary Antibody (green line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Negative control（white blue line）：PBS Isotype control（orange line）：Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.