| 产品编号 | bsm-52793R |
| 英文名称 | Rabbit Anti-TFRC antibody |
| 中文名称 | 转铁蛋白受体（CD71）重组兔单抗 |
| 别 名 | Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor (p90 CD71); TRFR; TFR1_HUMAN. |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 神经生物学 干细胞 糖尿病 细胞表面分子 淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 8G6 |
| 交叉反应 | Human,Rat (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 95kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Transferrin receptor |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] Function: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site. Subunit: Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Transferrin receptor protein 1, serum form: Secreted Post-translational modifications: N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. Similarity: Belongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain. SWISS: P02786 Gene ID: 7037 Database links: Entrez Gene: 7037 Human Entrez Gene: 22042 Mouse Omim: 190010 Human SwissProt: P02786 Human SwissProt: Q62351 Mouse Unigene: 529618 Human Unigene: 28683 Mouse Unigene: 98672 Rat |
| 产品图片 |
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: HT1080 (Human) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-CD71/Transferrin receptor (bsm-52793R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 100 kD
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD71 Transferrin receptor) Monoclonal Antibody, Unconjugated (bsm-52793R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD71 Transferrin receptor) Monoclonal Antibody, Unconjugated (bsm-52793R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TFRC) monoclonal Antibody, Unconjugated (bsm-52793R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
