| 产品编号 | bsm-54393R |
| 英文名称 | Rabbit Anti-PICK1 antibody |
| 中文名称 | 蛋白激酶Cα相互作用蛋白1重组兔单抗 |
| 别 名 | PICK 1; Pick1; PICK1_HUMAN; PRKCA binding protein; PRKCA-binding protein; Prkcabp; Protein interacting with C kinase 1; Protein interacting with PRKCA; Protein interacting with PRKCA 1; Protein kinase C alpha binding protein; Protein kinase C-alpha-binding protein; Protein that interacts with C kinase 1. |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 4G1 |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PICK1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. Function: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. Subunit: Monomer and homodimer. Subcellular Location: Cytoplasm, perinuclear region. Cell junction, synapse. Note=Also present at excitatory synapses. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. Similarity: Contains 1 AH domain. Contains 1 PDZ (DHR) domain. SWISS: Q9NRD5 Gene ID: 9463 Database links: Entrez Gene: 9463 Human Entrez Gene: 18693 Mouse Omim: 605926 Human SwissProt: Q9NRD5 Human SwissProt: Q62083 Mouse Unigene: 180871 Human |
| 产品图片 |
Sample:
Lane 1: SK-Br-3 cell lysate
Lane 2: 293 cell lysate
Primary: Anti-PICK1 (bsm-54393R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 46 kD
Observed band size: 52 kD
Blank control:293T.
Primary Antibody (green line): Rabbit Anti-PICK1 antibody (bms-54393R)
Dilution: 1:100 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1:1000.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
