| 产品编号 | Abk003 |
| 英文名称 | Rabbit Anti-EMT Research Antibody Assembly kit antibody |
| 中文名称 | EMT标志物抗体套装 |
| 别 名 | EMT Research Antibody Assembly kit(E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1) 上皮-间质转换(EMT)标志物抗体套装(E-Cadherin,N-Cadherin,Vimentin,Occludin,ZO-1); EMT标志物抗体套装 |
| 研究领域 | 肿瘤 细胞生物 细胞粘附分子 细胞表面分子 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | For research use only. Not intended for diagnostic or therapeutic use. |
| PubMed | PubMed |
| 产品介绍 |
Epithelial-mesenchymal transition (EMT) is a biological process that allows epithelial cell to assume mesenchymal phenotype. EMT and its reverse process, mesenchymal-epithelial transition (MET), are originally defined in the context of developmental stages and re-engaged in adults during wound healing, tissue regeneration, organ fibrosis, and cancer progression. Epithelial cells normally interacts with basement membrane via its basal surface. During EMT, they lose their cell polarity and cell-cell adhesion, and gain mesenchymal cell phenotype, including enhanced migratory capacity, invasiveness, elevated resistance to apoptosis, stem-like features, and increased production of ECM components. Accordingly, there is reprogrammed gene expression with EMT. Especially, some cytoskeleton proteins (such as cadherin family members), tight-junction proteins (such as Occludin and ZO-1) and intermediate filament of mesenchymal origin (such as Vimentin) undergo significant changes and serve as best EMT markers to evaluate EMT. EMT Research Antibody Assembly kit is one economical package of selected antibodies for most representative EMT markers, including E-Cadherin, N-Cadherin, Vimentin, Occludin, and ZO-1. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. EMT Research Antibody Assembly kit Rabbit Anti-E cadherin antibody (bs-1519R) Rabbit Anti-N-cadherin antibody (bs-1172R) Rabbit Anti-Vimentin antibody (bs-0756R) Rabbit Anti-Occludin antibody (bs-10011R) Rabbit Anti-ZO-1 antibody (bs-1329R) SWISS: N/A Gene ID: N/A Database links: EMT Research Antibody Assembly kit Rabbit Anti-E cadherin antibody (bs-1519R) Rabbit Anti-N-cadherin antibody (bs-1172R) Rabbit Anti-Vimentin antibody (bs-0756R) Rabbit Anti-Occludin antibody (bs-10011R) Rabbit Anti-ZO-1 antibody (bs-1329R)
上皮-间质转换(EMT)是一个使上皮细胞呈现间质细胞表型的生物学转变过程，EMT及其反向过程，间质-上皮转换(MET)，最初定义在早期发育阶段，并在成年期重新参与到伤口愈合、组织再生、器官纤维化和癌症进展等多种病理过程中。上皮细胞通常与基底膜紧密结合，在EMT过程中，它们失去细胞极性和细胞粘附并获得间质细胞表型，包括迁移能力、侵袭性、对凋亡的抗性增强、干细胞样特征和ECM组分的增加。与此相应很多基因表达也发生了重新编程，尤其是一些细胞骨架蛋白(如钙粘素家族成员)、紧密连接蛋白(如Occludin和ZO-1)和间质特有中间纤维(如Vimentin)发生了显著变化。因此，这些蛋白可作为评价EMT的最佳标志。 EMT标志物抗体套装极具性价比，包含了最具代表性的EMT标记物，包括E-cadherin、N-cadherin、Vimentin、Occludin和ZO-1。这些抗体在多种应用和物种中进行了测试。有关抗体具体使用信息，请参阅各个抗体产品数据表。 |
| 产品图片 |
Primary: Anti-Occludin (bs-10011R)
Paraformaldehyde-fixed, paraffin embedded (Normal skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated (bs-10011R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Primary: Anti- Vimentin (bs-0756R)
Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Primary: Anti- ZO-1 (bs-1329R)
Independently Validated Antibody, image provided by Science Direct, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.
Primary: Anti-N-cadherin (bs-1172R)
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
