| 产品编号 | bsm-54156R |
| 英文名称 | Rabbit Anti-CD11b antibody |
| 中文名称 | 整合素αM单克隆抗体 |
| 别 名 | Antigen CD11b (p170); CD11 antigen like family member B; CD11 antigen like family member C; Cell surface glycoprotein MAC 1 subunit alpha; CR 3 alpha chain; CR3A; Integrin alpha M; Integrin alpha X; Integrin, alpha M (complement component 3 receptor 3 subunit); Integrin, alpha X (antigen CD11C (p150), alpha polypeptide); Integrin, alpha X (complement component 3 receptor 4 subunit); ITGAM; ITGAX; Leu M5, alpha subunit; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor MO1; Leukocyte adhesion receptor p150,95; Leukocyte surface antigen p150,95, alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Myeloid membrane antigen, alpha subunit; Neutrophil adherence receptor alpha M subunit; p150 95 integrin alpha chain; SLEB6; ITAM_HUMAN; c equivalent; Integrin αM. |
| 研究领域 | 神经生物学 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 9B3 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 170kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant Human ITGAM |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] Function: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Subunit: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Interacts with JAM3. Subcellular Location: Plasma membrane Tissue Specificity: Predominantly expressed in monocytes and granulocytes. DISEASE: Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain. SWISS: P11215 Gene ID: 3684 Database links: Entrez Gene: 3684 Human Entrez Gene: 16409 Mouse Omim: 120980 Human SwissProt: P11215 Human SwissProt: P05555 Mouse Unigene: 172631 Human Unigene: 262106 Mouse |
| 产品图片 |
THP-1 (H) cells were treated with or without PMA (50 ng/mL) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with CD11b monoclonal antibody, unconjugated (bsm-54156R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
