产品编号 | bsk31002 |
英文名称 | Human LH ELISA Kit |
中文名称 | 人促黄体生成素酶联免疫试剂盒 |
种 属 | Human |
线性范围 | 0-200mIU/ml |
应用范围 | 人血清 |
检测限 | 2mIU/ml |
适用样品基质 | 人血清 |
保存条件 | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.). |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
This gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. 人促黄体生成激素(hLH)是由垂体前叶分泌的糖蛋白激素,其分子量大约为30,000,由两条多肽链(即α和β亚单位)组成。LH的α亚单位由89个氨基酸残基组成,并与FSH、TSH和 hCG 的α亚单位的结构相同,β亚单位在上述激素之间是不同的,从而赋予各激素各自的生物及免疫学特性。 在女性体中,LH在卵泡期与FSH一起促进卵泡的成熟、雌性激素的合成和分泌;促进排卵和使排卵后的卵泡转变为黄体。促进间质的生长。并促进黄体合成和孕激素与雌性激素的分泌。男性LH促进睾丸间质细胞增生,促进其合成和分泌睾酮。由于LH和FSH的作用是互相协同的,故二者常同时测定。 LH增高见于Klindfelter综合症,Turner综合症,性腺切除后及促性腺激素产生肿瘤;减低见于西蒙氏病,席汉氏综合症,肥胖性生殖器退化综合症,垂体性侏儒,睾丸肿瘤,卵巢肿瘤,神经性厌食及使用雌激素后。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |