| 产品编号 | bsm-54104R |
| 英文名称 | Rabbit Anti-phospho-MLKL (Ser345) antibody |
| 中文名称 | 磷酸化MLKL重组兔单抗 |
| 别 名 | phospho-MLKL(S345); p-MLKL(S345); MLKL(p-S345); hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain-like protein; mixed lineage kinase domain like pseudokinase; MLKL_HUMAN. |
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Specific References (1) | bsm-54104R has been referenced in 1 publications.
[IF=9.988] Ying Tu. et al. Developmental exposure to chlorpyrifos causes neuroinflammation via necroptosis in mouse hippocampus and human microglial cell line. ENVIRON POLLUT. 2022 Dec;314:120217 WB ; Mouse, Human.
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| 研究领域 | 细胞生物 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 7G4 |
| 交叉反应 | Human (predicted: Mouse) |
| 产品应用 | WB=1:500-2000,IP=1:10-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from mouse MLKL around the phosphorylation site of Ser345 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]. Function: Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Subunit: Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5. Subcellular Location: Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. Post-translational modifications: Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane. Similarity: Belongs to the protein kinase superfamily. Contains 1 protein kinase domain. SWISS: Q9D2Y4 Gene ID: 74568 Database links: Entrez Gene: 197259 Human Entrez Gene: 74568 Mouse Omim: 615153 Human SwissProt: Q8NB16 Human SwissProt: Q9D2Y4 Mouse Unigene: 119878 Human Unigene: 207971 Mouse Unigene: 105677 Rat |
| 产品图片 |
Western blot analysis of Phospho-MLKL (S345) on L929 cell lysates.
Lane 1 : L929 cells, whole cell lysate, 10 μg /lane.
Lane 2 : L929 cells were treated with 20 uM Z-VAD for 30 minutes, then added 20 ng/ml mTNF-alpha and 100 nM SM-164 for 4 hours, whole cell lysates, 10 μg/lane.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
