| 产品编号 | bsm-51320M |
| 英文名称 | Mouse Anti-TIMP-2 antibody |
| 中文名称 | 金属蛋白酶组织抑制因子-2单克隆抗体 |
| 别 名 | Metalloproteinase inhibitor 2 precursor; Tissue inhibitor of metalloproteinases 2; Collagenase inhibitor; CSC 21K; CSC21K; TIMP2; TIMP 2; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP2_HUMAN; Metalloproteinase inhibitor 2; CSC-21K; TIMP-2. |
| 研究领域 | 肿瘤 心血管 细胞生物 神经生物学 信号转导 细胞凋亡 合成与降解 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 4C9 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:20-100,IHC-F=1:20-100,Flow-Cyt=1:20-50,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 24kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TIMP-2 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]. Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Subunit: Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity. Subcellular Location: Secreted. Post-translational modifications: The activity of TIMP2 is dependent on the presence of disulfide bonds. Similarity: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain. SWISS: P16035 Gene ID: 7077 Database links: Entrez Gene: 100135629 Guinea pig Entrez Gene: 7077 Human Entrez Gene: 21858 Mouse Entrez Gene: 100008689 Rabbit Omim: 188825 Human SwissProt: Q9WUC6 Guinea pig SwissProt: P16035 Human SwissProt: P25785 Mouse SwissProt: Q9TRZ7 Rabbit Unigene: 633514 Human Unigene: 206505 Mouse Unigene: 10161 Rat |
| 产品图片 |
Sample:
Lane 1: Human SW480 cell lysates
Primary: Anti- TIMP-2 (bsm-51320M) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD
Sample:
Lane 1: Human HT-1080 cell lysates
Primary: Anti- TIMP-2 (bsm-51320M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (human kidney sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-2) Monoclonal Antibody, Unconjugated (bsm-51320M) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Blank control:K562.
Primary Antibody (green line): Mouse Anti-TIMP-2 antibody (bsm-51320M)
Dilution:1:25;
Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution:1:400.
Protocol
The cells were fixed with 2% PFA (10min at room temperature)and then permeabilized with 90% methanol for 10 min.The cells were then incubated in 2%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
