| 产品编号 |
C5076 |
| 英文名称 |
Lowry Protein Assay Kit |
| 中文名称 |
Lowry法蛋白浓度测定试剂盒（1000T） |
| 别 名 |
Protein Quantitation Assay; Modified Lowry Protein Assay Kit; Protein Quantitation Assay; 稳定型Lowry法蛋白浓度测定试剂盒; 改良型Lowry法蛋白浓度测定试剂盒 |
| 保存条件 |
试剂盒2-8℃保存，标准品-20℃保存，开封使用后请密封保存 |
| 注意事项 |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The folin phenol reagent method consists of two steps: the first step is protein interact with copper to form protein-copper complex in an alkaline solution. The second step is the complex reduce the Folin reagent to produce dark blue. The amount of color produced is proportional to protein concentration. The quantitative rang of protein is 5~100μg/ml. Folin reagent chromogenic reaction is caused by tyrosine, tryptophan and cysteine. Therefore, phenols, citric acid and sulfhydryl compounds in the sample have interfere. Besides, different proteins have different color intensity due to different contents of tyrosine and tryptophan.
Lowry法包括两步反应：第一步是在碱性条件下，蛋白质与铜作用生成蛋白质-铜络合物；第二步是此络合物将Folin试剂还原，产生深蓝色，颜色深浅与蛋白质含量成正比。定量范围为5～100μg/ml蛋白质。Folin试剂显色反应由酪氨酸、色氨酸和半胱氨酸引起，因此样品中若含有酚类、柠檬酸和巯基化合物均有干扰作用。此外，不同蛋白质因酪氨酸、色氨酸含量不同而使显色强度稍有不同。
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