| 产品编号 | bs-60295G-AF488 |
| 英文名称 | AF488 AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) |
| 中文名称 | AF488标记山羊抗兔IgG(H+L), F(ab')2片段抗体 |
| 别 名 | Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L); F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (AF488); F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/AF488; F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (AF488); AF488 AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L); |
| 克隆类型 | |
| 交叉反应 | Rabbit |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 亚 型 | |
| 保存条件 | Store at -20 ℃. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Target: Rabbit Host: Goat Antibody Format: F(ab')? Fragment Specificity: IgG (H+L) Minimal Cross Reactivity: Human, Mouse, Rat Serum Proteins Conjugate: Alexa Fluor? 488 (Amax: 493 Emax: 519nm) Product Category: F(ab')? Fragment Affinity-Purified Antibodies Dilution Range: 1:100-800 for most applications Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative: 0.05% Sodium Azide Information: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species. F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
