| 产品编号 | bs-22181R |
| 英文名称 | Integrin alpha 4 Rabbit pAb |
| 中文名称 | 整合素α4（CD49d）抗体 |
| 别 名 | alpha 4 subunit of VLA 4 receptor; ITG-α4: Antigen CD49D; CD 49d; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha IV; ITGA 4; ITGA4; LPAM23; MGC90518; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor alpha 4 Subunit; VLA 4; VLA4; integrin alpha-4; ITA4_HUMAN; |
| 研究领域 | 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 111 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Integrin alpha 4 : 191-290/1032 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] Function: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Subunit: Heterodimer of an alpha and a beta subunit. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylation on Ser-1027 inhibits PXN binding. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. SWISS: P13612 Gene ID: 3676 Database links: Entrez Gene: 3676 Human Entrez Gene: 16401 Mouse Omim: 192975 Human SwissProt: P13612 Human SwissProt: Q00651 Mouse Unigene: 440955 Human Unigene: 31903 Mouse 整合素-α-4型 Integrin α4 (CD49d)整合素-α是介导细胞与细胞外基质粘附作用的主要因子。 |
| 产品图片 |
Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti- Semaphorin 5B (bs-22181R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140/68 kD Observed band size: 140/68 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
