产品编号 | C07-01006 |
英文名称 | Ac-LEVD-pNa |
中文名称 | Caspase 4显色底物 (Ac-LEVD-pNa) |
别 名 | Ac-Leu-Glu-Val-Asp-pNA; Caspase 4 Substrate (ICH-2), chromogenic; Caspase-4 Substrate; Caspase Substrate, chromogenic; Ac-LEVD-pNA (Caspase 4显色底物); Ac-LEVD-pNA,Caspase 4显色底物; |
保存条件 | Store at -20 °C for one year. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Caspase 4, a cysteine protease activator of inflammatory processes, may be detected using a variety of chromogenic and fluorogenic peptide substrates built around the LEVD (leu-glu-val-asp) sequence such as the chromogenic substrate Ac-LEVD-pNa (Acetyl-Leu-Glu-Val-Asp-p-nitroanalide).
级别/纯度:BR, >95%(HPLC) 分子式:C26H34N6O13 分子量:638.58 序列:N-Acetyl-Leu-Glu-Val-Asp-p-Nitroanilide 来源:合成 溶解性:Soluble in DMSO 敏感性:对光敏感 简介:Ac-LEVD-pNA是Caspase 4的底物,被剪切后可产生黄色的pNA (p-nitroaniline),pNA在405nm附近有强吸收,可以通过测定吸光度来检测Caspase 4的活性。 用途:用作半胱氨酸的天冬氨酸蛋白水解酶显色底物。 配方:20mM in DMSO: 5mg加入0.39ml DMSO,或每12.73mg加入1ml DMSO,配制成20mM溶液。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |