产品编号 | C07-01004 |
英文名称 | Ac-IETD-pNA |
中文名称 | Caspase 8显色底物 (Ac-IETD-pNA) |
别 名 | N-Acetyl-Ile-Glu-Thr-Asp-p-nitroanilide; Caspase-8 Substrate; N-Acetyl-Ile-Glu-Thr-Asp-pNA; Caspase-8 Substrate(Chromogenic); N-Acetyl-Ile-Glu-Thr-Asp-pNA; Caspase Substrate, chromogenic; Ac-IETD-pNA (Caspase 8显色底物); Caspase 8显色底物,Ac-IETD-pNA; |
保存条件 | Store at -20 °C for one year. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Chromogenic substrate for caspase 8 and granzyme B. IETD is the sequence of amino acid residues 172-175 of procaspase 3, which is cleaved during apoptosis to produce the p12 subunit and a p20 peptide that is further cleaved to form the p17 subunit of mature caspase 3. 纯度:>95%(HPLC) 分子式:C34H37N9O11 分子量:747.7 序列:N-Acetyl-Ile-Glu-Thr-Asp-p-Nitroanilide 来源:合成 外观/性状:Lyophilized Yellow Powder 溶解性:Soluble in DMSO 敏感性:对光敏感 简介:Ac-IETD-pNA是Caspase 8的底物,被剪切后可产生黄色的pNA (p-nitroaniline),pNA在405nm附近有强吸收,可以通过测定吸光度来检测Caspase 8的活性。 用途:Chromogenic substrate for caspase-1, -4 and -5. Most potent for caspase-1. ; max of pNA 400nm. 配方:20mM in DMSO: 5mg加入0.39ml DMSO,或每12.77mg加入1ml DMSO,配制成20mM溶液。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |