[IF=4.932] Yi Ren. et al. Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway. Int Immunopharmacol. 2021 Nov;100:108077  WB ;  Mouse.  

PubMed:34464887

Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: HUVEC (Human) Cell Lysate at 30 ug
Lane 3: A431 (Human) Cell Lysate at 30 ug
Lane 4: LOVO (Human) Cell Lysate at 30 ug
Lane 5: SiHa (Human) Cell Lysate at 30 ug
Primary: Anti-CD144/VE Cadherin (bs-22363R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD

Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HUVEC cell lysates
Lane 3: Human A431 cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-CD144/VE Cadherin (bs-22363R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 140 kDa

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD144/VE Cadherin) Polyclonal Antibody, Unconjugated (bs-22363R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD144/VE Cadherin) Polyclonal Antibody, Unconjugated (bs-22363R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control:HUVC.
Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (bs-22363R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control:HUVEC.
Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (bs-22363R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.