| 产品编号 | bsm-33432M |
| 英文名称 | HSP40 Mouse mAb |
| 中文名称 | 热休克蛋白家族40单克隆抗体 |
| 别 名 | HSP40 homolog; DnaJ(Hsp40) homolog subfmaily B member 1; DNAJ 1; DNAJ B1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB 1; Dnajb1; DNAJB1 protein; DNJB1_HUMAN; HDJ 1; HDJ-1; HDJ1; Heat shock 40 kDa protein 1; Heat shock 40kD protein 1; Heat shock protein 40; Hsp 40; HSP40; HSPF 1; HSPF1; Human DnaJ protein 1; Radial spoke 16 homolog B; RSPH16B; Sis1. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 3G2 |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HSP40 Protein: 1-77/314 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
DNAJB4 is a member of the evolutionarily conserved DNAJ/HSP40 protein family. Function: Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Subunit: Homodimer. Subcellular Location: Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. Tissue Specificity: Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver. Similarity: Contains 1 J domain. SWISS: P25685 Gene ID: 3337 Database links: Entrez Gene: 3337 Human Entrez Gene: 81489 Mouse Omim: 604572 Human SwissProt: P25685 Human SwissProt: Q9QYJ3 Mouse Unigene: 515210 Human Unigene: 282092 Mouse Unigene: 128947 Rat |
| 产品图片 |
Sample:
U251(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti- HSP40 (bsm-33432M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- HSP40 (bsm-33432M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
