| 产品编号 | C01-01014 |
| 英文名称 | HEPES buffer, 2×(pH7.2-7.4, sterile) |
| 中文名称 | Hepes Buffer (2×,pH7.2-7.4,无菌) |
| 别 名 | 2×HEPES buffer(pH7.2-7.4，sterile); 2 × HEPES buffer(pH7.2-7.4，sterile); 2-[4-(羟乙基)-1-哌嗪基]乙磺酸;N-(2-羟乙基)哌嗪-N'-乙烷磺酸;4-羟乙基哌嗪乙磺酸;N-(2-羟乙基)哌嗪-N'-2-乙烷磺酸;4-(2-羟乙基)-1-哌嗪乙磺酸;N-2-羟乙基哌嗪-N'-2-乙磺酸(HEPES);羟乙基哌嗪乙磺酸;2-[4-(2-羟乙基)-1-哌嗪乙烷]磺酸; 2×HEPES(pH7.2- 7.4); HEPES buffer, 2X(pH7.2-7.4, sterile); |
| 保存条件 | Store at 2-8℃ for one year. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Application HEPES has been used as a component of: Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices. Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity. General description: HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. Hepes是一种氢离子缓冲剂，能较长时间控制恒定的pH范围，有效缓冲范围为pH6.8-8.2。可用于配制蛋白提取缓冲液、细胞培养用缓冲液等，对细胞无毒性作用。使用终浓度为10-50mmol/L，一般培养液内含20mmol/LHEPES即可达到缓冲能力，细胞培养常用终浓度为 5-30mM/L。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
