| 产品编号 | bsm-30105M |
| 英文名称 | Mouse Anti-human CD90/Thy-1 antibody |
| 中文名称 | 小鼠抗人CD90单克隆抗体 |
| 别 名 | CD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN. CD7; CD90 antigen; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; |
| 研究领域 | 细胞生物 免疫学 神经生物学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 2D9 |
| 交叉反应 | Human |
| 产品应用 | Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12kDa |
| 检测分子量 | 25-35 |
| 性 状 | Liquid |
| 浓 度 | 0.5mg/ml |
| 免 疫 原 | HEL cells |
| 亚 型 | Mouse IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M PBS (pH7.4). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] Function: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Abundant in lymphoid tissues. Post-translational modifications: Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P04216 Gene ID: 7070 Database links: Entrez Gene: 7070 Human Entrez Gene: 21838 Mouse Omim: 188230 Human SwissProt: P04216 Human SwissProt: P01831 Mouse Unigene: 644697 Human Unigene: 3951 Mouse Unigene: 108198 Rat |
| 产品图片 |
Blank control:HepG2.
Primary Antibody (green line): Mouse Anti- CD90 antibody (bsm-30105M)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Secondary Antibody : Goat anti-Mouse IgG-CY5
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
