产品编号 | bs-23824R |
英文名称 | Rabbit Anti-B4GALT6 antibody |
中文名称 | B4GALT6抗体 |
别 名 | Beta-1,4-Galactosyltransferase 6; UDP-Galactose:Beta-N-Acetylglucosamine Beta-1,4-Galactosyltransferase 6; UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase, Polypeptide 6; UDP-Gal:Glucosylceramide Beta-1,4-Galactosyltransferase; UDP-Gal:Beta-GlcNAc Beta-1,4-Galactosyltransferase 6; Beta-1,4-GalTase 6; Beta4Gal-T6; B4Gal-T6; Beta4GalT-VI; B4GT6_HUMAN; |
研究领域 | 细胞生物 合成与降解 糖蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 45kDa |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human B4GALT6 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis. [provided by RefSeq, Jul 2008] Function: Required for the biosynthesis of glycosphingolipids. Subcellular Location: Golgi apparatus Tissue Specificity: High expression in brain and adrenal gland, lower in liver, lung, colon and peripheral white blood cells. Similarity: Belongs to the glycosyltransferase 7 family. SWISS: Q9UBX8 Gene ID: 9331 Database links: Entrez Gene: 9331 Human Entrez Gene: 56386 Mouse Omim: 604017 Human SwissProt: Q9UBX8 Human SwissProt: Q9WVK5 Mouse Unigene: 591063 Human Unigene: 393827 Mouse |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B4GALT6) Polyclonal Antibody, Unconjugated (bs-23824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B4GALT6) Polyclonal Antibody, Unconjugated (bs-23824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |