| 产品编号 | bs-23550R |
| 英文名称 | NCR1 Rabbit pAb |
| 中文名称 | 细胞毒性受体NK-p46抗体 |
| 别 名 | Natural killer cell p46-related protein; Natural cytotoxicity triggering receptor 1; NK-p46; NKp46; NK cell-activating receptor; Lymphocyte antigen 94 homolog; CD335; CD 335; LY94; NCTR1_HUMAN. |
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Specific References (1) | bs-23550R has been referenced in 1 publications.
[IF=7.658] Xin Fang. et al. IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10. Pharmacol Res. 2022 Mar;177:106132 IHC ; Mouse.
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| 研究领域 | 肿瘤 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NCR1: 1-100/304 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. NCR1 is a glycoprotein that has two extracellular Ig-like domains followed by a ~40 amino acid residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. NCR1 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. NCR1 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines. Function: Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Subunit: Interacts with CD247 and FCER1G. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Selectively expressed by both resting and activated NK cells. Similarity: Belongs to the natural cytotoxicity receptor (NCR) family. Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: O76036 Gene ID: 9437 Database links: Entrez Gene: 9437 Human Entrez Gene: 17086 Mouse Omim: 604530 Human SwissProt: O76036 Human SwissProt: Q8C567 Mouse Unigene: 97084 Human Unigene: 240231 Mouse Unigene: 30049 Rat NKp46是很重要的的自然细胞毒受体，在自然杀伤作用中起关键作用。细胞毒性受体家族（NCRs）主要有：NKp46、NKp44和NKp30，其中NKp46和NKp30表达于所有静止和活化的NK细胞，而NKp44则选择性的表达于活化的NK细胞。靶细胞上的配体分子与NCRs的结合（交联）引发NK细胞的活化导致靶细胞的裂解和大量细胞因子的产生。现已确认NKp46和NKp44可以识别病毒上的配体分子红血球凝聚素，而NKp46则能识别肿瘤细胞上的乙酰硫酸多聚糖。 |
| 产品图片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- NCR1 (bs-23550R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 46 kD
Sample: A431 (Human) Cell Lysate at 30 ug Raji (Human) Cell Lysate at 30 ug Primary: Anti- NCR1 (bs-23550R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30m
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-NCR1 antibody (bs-23550R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Pr
Blank control:Mouse kidney.
Primary Antibody (green line): Rabbit Anti-NCR1 antibody (bs-23550R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Pr
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
