| 产品编号 | D-9801 |
| 英文名称 | FITC |
| 中文名称 | 荧光标记染料FITC/异硫氰酸荧光素酯 |
| 别 名 | Fluorescein isothiocyanate isomer I; 3',6'-dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; 5-FITC; FITC-I 绿色荧光素 |
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Specific References (5) | D-9801 has been referenced in 5 publications.
[IF=9.229] Seonhwa Lee. et al. Nanoparticle-Enabled Multiplexed Electrochemical Immunoassay for Detection of Surface Proteins on Extracellular Vesicles. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX FC ; Human.
[IF=8.713] Peng Yang. et al. Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes. ELIFE. 2022 Oct Other ; Other.
[IF=5.763] You Shuang. et al. Recombinant humanized collagen remodels endometrial immune microenvironment of chronic endometritis through macrophage immunomodulation. REGEN BIOMATER. 2023 Apr;:
[IF=3.119] Tan F et al. Identification and initial functional characterization of lysosomal integral membrane protein type 2 (LIMP-2) in turbot (Scophthalmus maximus L.). Dev Comp Immunol. 2019 Oct;99:103412. Other ;
[IF=0] Shen YY et al. Novel C-type lectin from razor clam Sinonovacula constricta agglutinates bacteria and erythrocytes in a Ca -dependent manner.Developmental & Comparative Immunology, 86, 9–16. other ;
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| 保存条件 | Store at -20℃, protect from light. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed. FITC分子式：
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
