产品编号 | C02-01001 |
英文名称 | Poly-L-Lysine Solution |
中文名称 | 多聚赖氨酸溶液(10×防脱片剂) |
别 名 | Poly-L-lysine; PLL; PLL Solution; Poly-L-lysine hydrobromide; 多聚赖氨酸溶液(10×防脱片剂); 多聚赖氨酸溶液粘贴剂; Poly-L-lysine/多聚赖氨酸; 聚-L-赖氨酸; 多聚赖氨酸; 多聚- L- 赖氨酸(7- 15万 ); |
Specific References (1) | C02-01001 has been referenced in 1 publications.
[IF=1.287] Xuefeng X et al. Epithelial–mesenchymal transition and metastasis of colon cancer cells induced by the FAK pathway in cancer-associated fibroblasts. J Int Med Res. 2020 Jun;48(6):300060520931242. IHC ;
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保存条件 | Store at 2-8℃. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Poly-L-Lysine solution is intended for use as an adhesive subbing solution for immunohistochemistry(IHC) and routine histologic staining preparations. The loss of paraffin and frozen sections from slides has long been a problem during routine Histologic staining procedures. Various adhesives including albumin, gelatin and chrome alum have been applied to slides to minimize this loss. Different solutions of Poly-L-Lysine have been shown to be most effective in promoting adhesive of sections. The polycationic nature of this molecule allows interaction with the anionic sites of tissue sections resulting in strong adhesive properties. Poly-L-Lysine has been demonstrated as an effective tissue adhesive for use in various microwave procedures. 简介 多聚赖氨酸溶液是广泛应用的组织切片与玻片黏合剂,该多聚阳离子分子与组织切片上的阴离子相互作用会产生较强的黏合力。 适用组织学,免疫组织化学,冰冻切片,细胞涂片,原位杂交等使用的玻片的防脱片处理,以防实验操作过程中组织掉片。也可用于细胞培养,增加细胞贴壁能力。 Poly-L-lysine的中文名为多聚赖氨酸,简称PLL。本Poly-L-lysine为Poly-L-lysine hydrobromide,分子式为L-Lys-(L-Lys)n-L-Lys·xHBr,分子量为150,000-300,000,CAS Number 25988-63-0。 本产品为进口分装。分子量大于70,000的多聚赖氨酸可以用于促进细胞贴壁生长,本产品可以用于促进细胞的贴壁生长。本产品也可以用于组织学(Histology)分析时的粘片剂。 使用说明: 1. 灭菌的蒸馏水1:10稀释该多聚赖氨酸溶液。 2. 用之前将稀释的多聚赖氨酸溶液放在室内,使其温度到室温18-26℃ 3. 将玻片浸在稀释的多聚赖氨酸溶液5分钟。注意增加时间不会提高包被效果。 4. 在60°C烘箱1小时干燥,或室温18-26℃过夜干燥待用。 注意事项: 1. 每100ml已稀释的多聚赖氨酸溶液要包被的玻片40-90张,超过90张片子将影响其黏合力。 2. 用之前的玻片必须保持清洁。必要时用含1% HCl的70%乙醇溶液来清洗。 3. 不要在用过的稀释液中加新的溶液。 4. 释过的多聚赖氨酸溶液要放在2-8℃,至少在3个月内是稳定的。 5. 用过的稀释液要过滤,若出现浑浊或长菌要丢弃。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |