产品编号 | C01-03001 |
英文名称 | Normal Goat Serum (10%) |
中文名称 | 10%封闭山羊血清 |
别 名 | 10% Normal Goat Serum; Blocking Buffer for Immunol Staining; Normal Goat Serum Blocking Buffer; blocking goat serum; blocking grade goat serum; 10% NGS, goat serum blocking buffer; 免疫染色封闭液; 免疫组化封闭液; 免疫组化染色封闭液; HIC封闭液; 10%山羊血清; 10% 山羊血清; 10%山羊血清封闭液; 正常山羊血清; |
Specific References (16) | C01-03001 has been referenced in 16 publications.
|
|
保存条件 | Store at 2-8℃. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Goat Serum blocking reagent or NGS can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications. Application: Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding. 封闭血清适用于免疫组化或者免疫荧光实验中样本的封闭。可以封闭待检样本中与蛋白质非特异性结合的位点,另外还可以封闭样品中内源性的Fc片段结合位点,阻断抗体与样品中的Fc受体结合,从而降低背景,减少假阳性。在选择封闭血清时,要注意封闭用血清中不能含有目标蛋白,且与一抗来源不同,可用与二抗相同来源的封闭血清。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |