| 产品编号 | BA00103 |
| 英文名称 | Annexin V-AF647 Apoptosis Detection Kit |
| 中文名称 | Annexin V-Alexa Fluor 647细胞凋亡检测试剂盒 |
| 别 名 | Annexin V-AF647/PI Apoptosis Detection Kit; Annexin V-AF647 Apoptosis Detection Kit; Annexin V-Alexa Fluor 647/PI Kit; Annexin V-Alexa Fluor 647/PI Kit; Annexin V-AF647/PI Kit; Annexin V-AF647/PI双染细胞凋亡检测试剂盒; Annexin V-AF647细胞凋亡检测试剂盒; AF647 Annexin V/PI细胞凋亡检测试剂盒; Annexin V-AF647/PI双染细胞凋亡检测试剂盒; ANNEXIN V- AF647凋亡检测试剂盒; Annexin V-AF647/PI细胞凋亡检测试剂盒; 细胞凋亡检测试剂盒; 细胞凋亡试剂盒; |
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Specific References (2) | BA00103 has been referenced in 2 publications.
[IF=10.164] Wang D et al. Loss of 4.1N in epithelial ovarian cancer results in EMT and matrix-detached cell death resistance.. Protein Cell. 2020 May 25. FCM ; Human.
[IF=5.714] Xiangming Liu. et al. Taxifolin ameliorates cigarette smoke-induced chronic obstructive pulmonary disease via inhibiting inflammation and apoptosis. INT IMMUNOPHARMACOL. 2023 Feb;115:109577
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| 保存条件 | Store at 4℃. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
磷脂酰丝氨酸（PS）是一种带负电荷的磷脂，正常细胞中，PS只分布在细胞膜脂质双层的内侧，而在细胞凋亡早期，细胞膜 PS由脂膜内侧翻向细胞膜外侧，使PS暴露在细胞膜外表面。Annexin V是一种分子量为35~36kD 的Ca2+依赖性磷脂结合蛋白，与磷脂酰丝氨酸有高度亲和力，故可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。因此Annexin V被公认为检测细胞早期凋亡的灵敏指标之一。 将Annexin V进行Alexa Fluor 647标记，以标记了的Annexin V作为探针，利用荧光显微镜或流式细胞仪可检测细胞凋亡的发生。碘化丙啶（Propidium Iodide, PI）是一种核酸染料，它不能透过正常细胞或早期凋亡细胞的完整的细胞膜，但对凋亡中晚期的细胞和坏死细胞，PI能够透过细胞膜而使细胞核染红。因此采用Annexin V与PI双染的方法，就可以将处于不同凋亡时期的细胞区分开来。 |
| 产品图片 |
Jurkat cells (T-cell leukemia,human) treated with 10 μM of camptothecin for 4 hours(panel right) or untreated control(panel left).
1. Wash cells twice with cold PBS and then resuspend cells in 1×Binding Buffer at a concentration of 1×106 cells/ml. 2. Transfer 100 μl of the solution (1×105cells) to a 5 ml culture tube. 3. Add 5 μl of Annexin V-AF647. 4. Add 5 μl PI. 5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 6. Add 400 μl of 1×Binding Buffer to each tube.Analyze by flow cytometry within 1 hr. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
