产品编号 | C05-07001 |
英文名称 | Western Antibody Diluent (TBS) |
中文名称 | 通用型WB抗体稀释液(TBS) |
别 名 | Western Primary Antibody Diluent; Antibody Diluent; Primary Antibody Dilution Buffer for Western Blot; Western Primary Antibody Dilution Buffer; Antibody Diluent(TBST); Western一抗稀释液; Western一抗稀释缓冲液; Western抗体稀释液; WB抗体稀释液; Western blotting 专用一抗二抗稀释液; 通用型WB抗体稀释液 |
Specific References (3) | C05-07001 has been referenced in 3 publications.
[IF=6.953] Chao Xin. et al. The novel nanocomplexes containing deoxycholic acid-grafted chitosan and oleanolic acid displays the hepatoprotective effect against CCl4-induced liver injury in vivo. Int J Biol Macromol. 2021 Aug;185:338 WB ;
[IF=4.501] Fang Shi. et al. Phosphatidylinositol 3-Kinase/Protein Kinase B/Mammalian Target of the Rapamycin Pathway-Related Protein Expression in Lung Squamous Cell Carcinoma and Its Correlation with Lymph Node Metastasis.. J ONCOL. 2022 Aug;2022:4537256-4537256 Other ; Other.
[IF=3.115] Yongfang Ou. et al. Dietary Ochratoxin A Contamination Modulates Oxidative Stress, Inflammation Processes and Causes Fibrosis in in vitro and in vivo Lung Models. FRONT BIOSCI-LANDMRK. 2023 Feb;28(2):22
|
|
保存条件 | 2-8℃保存,一年有效。. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
产品简介: 通用型WB抗体稀释液(Western Antibody Dilution)适用于免疫印迹实验中一抗二抗的稀释。本产品添加了抗体保护剂、稳定剂和防腐剂等成分,能有效减少一抗的非特异性结合,增加了稀释后抗体的稳定性。用此稀释液稀释后的一抗二抗可在2-8℃环境下稳定一周。 使用方法: 参考一抗二抗的使用说明,以及样品中目的蛋白的含量或个人优化条件,按照适当比例将适量抗体加入抗体稀释液中,充分混匀后即可使用。抗体最好以浓缩液形式保存,现用现稀释,稀释后的抗体可2-8℃保存建议不要超过一周。 注意事项: 1.如需抗体回收再利用,建议在2-8℃保存,以用于下次的 Western实验。进行抗体的结合反应时可适当补充抗体,建议尽量在 4℃进行一抗和蛋白膜的结合反应,以减缓一抗的衰减速度。 2.如果发现有沉淀或细菌污染请弃用。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |