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BCA Protein Assay Kit (C05-02001)  
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说明书: 下载
40ml(200T)/180.00元
100ml(500T)/318.00元
100ml×10(5000T)/2400.00元
大包装/询价

产品编号 C05-02001
英文名称 BCA Protein Assay Kit
中文名称 BCA蛋白定量试剂盒
别    名 BCA Kit; bicinchoninic acid; BCA Assay Kit; Bicinchoninic Acid Kit for Protein Determination; BCA Protein Quantitative Kit;   BCA蛋白浓度测定试剂盒; BCA法蛋白质含量测定; BCA蛋白质定量试剂盒; BCA蛋白质含量试剂盒; BCA蛋白质含量检测试剂盒; BCA蛋白定量试剂盒; BCA蛋白定量试剂盒; BCA试剂盒; 蛋白含量测定; BCA 蛋白定量分析试剂盒;
Specific References  (35)     |     C05-02001 has been referenced in 35 publications.
[IF=13.281] Qin Ouyang. et al. Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease. 2022 Feb 19  Other ;  Other.  
[IF=10.107] Zhao Yin. et al. Discovery of Berberine that Targetedly Induces Autophagic Degradation of both BCR-ABL and BCR-ABL T315I through Recruiting LRSAM1 for Overcoming Imatinib Resistance. Clin Cancer Res. 2020 Aug;26(15):4040-4053  other ;  
[IF=9.417] Wen Zhang. et al. Construction of an ornidazole/bFGF-loaded electrospun composite membrane with a core-shell structure for guided tissue regeneration. MATER DESIGN. 2022 Sep;221:110960  
[IF=8.911] Yin Z et al. Discovery of berberine that targetedly induce autophagic degradation of both BCR-ABL and BCR-ABL T315I through recruiting LRSAM1 for overcoming imatinib-resistance. Clin Cancer Res. 2020 Feb 25.  Other ;  
[IF=7.666] Weiheng Zhang. et al. Long-Term Treatment with Gadopentetic Acid or Gadodiamide Increases TRPC5 Expression and Decreases Adriamycin Nuclear Accumulation in Breast Cancer Cells. CELLS-BASEL. 2023 Jan;12(9):1304  
[IF=7.31] Zitong Ma. et al. Epimedii Folium and Ligustri Lucidi Fructus Promote Osteoblastogenesis and Inhibit Osteoclastogenesis against Osteoporosis via Acting on Osteoblast-Osteoclast Communication. OXID MED CELL LONGEV. 2023 Jan 31;2023:7212642  
[IF=7.035] Jialiang Wei. et al. Protective role of TLR9-induced macrophage/microglia phagocytosis after experimental intracerebral hemorrhage in mice. CNS NEUROSCI THER. 2022 Jul;:  
[IF=6.706] Zefu Chen. et al. Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins. NUTRIENTS. 2022 Jan;14(24):5222  
[IF=6.638] Wang Z et al. Light-activatable dual prodrug polymer nanoparticle for precise synergistic chemotherapy guidedby drug-mediated computed tomography imaging. Acta Biomater. 2019 May 22.  Other ;  
[IF=6.208] Haibo Yao. et al. Whole-Transcriptome Sequencing of Antler Tissue Reveals That circRNA2829 Regulates Chondrocyte Proliferation and Differentiation via the miR-4286-R+1/FOXO4 Axis. INT J MOL SCI. 2023 Jan;24(8):7204  
[IF=5.738] Gao Xiaoqiang. et al. Regulation of Gamma-Aminobutyric Acid Transaminase Expression and Its Clinical Significance in Hepatocellular Carcinoma. FRONT ONCOL. 2022 Jun;0:2857  
[IF=5.56] Wang Haoxia. et al. Influences of flavonoids from Sedum aizoon L. on biofilm formation of Pseudomonas fragi. APPL MICROBIOL BIOT. 2023 Apr;:1-11  
[IF=5.455] Qin, Gaofeng. et al. Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro. Chin Med-Uk. 2021 Dec;16(1):1-19  WB ;  Mouse.  
[IF=5.195] Zhangbi Wu. et al. Ginsenoside-Rg1 mitigates cardiac arrest-induced cognitive damage by modulating neuroinflammation and hippocampal plasticity. EUR J PHARMACOL. 2023 Jan;938:175431  
[IF=4.962] Yingying Chen. et al. SUZ12 participates in the proliferation of PNH clones by regulating histone H3K27me3 levels. 2022 Jan 06  Other ;  Other.  
[IF=4.547] Li Jie. et al. S100A9 plays a key role in Clostridium perfringens beta2 toxin-induced inflammatory damage in porcine IPEC-J2 intestinal epithelial cells. BMC GENOMICS. 2023 Dec;24(1):1-14  
[IF=3.974] Wei H et al. Alveolar macrophage reaction to PM2. 5 of hazy day in vitro: Evaluation methods and mitochondrial screening to determine mechanisms of biological effect.Ecotoxicol Environ Saf. 2019 Jun 15;174:566-573.  Other ;  
[IF=3.69] Zitong Ma. et al. Comprehensive evaluation of the combined extracts of Epimedii Folium and Ligustri Lucidi Fructus for PMOP in ovariectomized rats based on MLP-ANN methods. J Ethnopharmacol. 2021 Mar;268:113563  WB ;  
[IF=3.654] Mengying Zhang. et al. Preparation of Black pepper (Piper nigrum L.) essential oil nanoparticles and its antitumor activity on triple negative breast cancer in vitro. J FOOD BIOCHEM. 2022 Sep;:e14406  Other ;  Other.  
[IF=3.575] Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 Dec;:  WB ;  Human.  
[IF=3.53] Adil Hassan et al. Anti-atherosclerotic effects of Lactobacillus plantarum ATCC 14917 in ApoE −/− mice through modulation of proinflammatory cytokines and oxidative stress. Appl Microbiol Biotechnol. 2020 Jul;104(14):6337-6350.  WB ;  Mouse.  
[IF=3.399] SiZhe Yu. et al. NT157 Inhibits HCC Migration via Downregulating the STAT3/Jab1 Signaling Pathway:. Technol Cancer Res T. 2021;():  WB ;  Human.  
[IF=3.318] Songsong Kang. et al. Tandem mass tag-based proteomics analysis reveals the effects of Guri Gumu-13 pill on drug-induced liver injury. J CHROMATOGR B. 2022 Jun;:123353  
[IF=3.241] Tang X et al. Evaluation of CaD on bone structure and bone metabolic changes in senile osteoporosis rats based on MLP-ANN methods. Food Funct. 2019 Nov 25.  Other ;  
[IF=3.093] Guo Xiao. et al. Effects of Transcutaneous Auricular Vagus Nerve Stimulation on Peripheral and Central Tumor Necrosis Factor Alpha in Rats with Depression-Chronic Somatic Pain Comorbidity. Neural Plast. 2020;2020:8885729  
[IF=3.061] Jie Li. et al. Decreased S100A9 expression alleviates Clostridium perfringens beta2 toxin-induced inflammatory injury in IPEC-J2 cells. PEERJ. 2023 Jan;11:e14722  
[IF=2.959] Peng W et al.Expression of osteoprotegerin and receptor activator for the nuclear factor-κB ligand in XACB/LV-bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis.(2018) J. Cell. Biochem. Oct 18  WB ;  
[IF=2.532] Jianchao Ren. et al. Ejaculatory Duct Obstruction Affects Seminal Vesicle Contractile Efficacy and Smooth Muscle Ultrastructure in a Rat Model. ANDROLOGIA. 2023;2023:5022466  
[IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
[IF=2.031] Yujing Wang. et al. SIRT1 promotes proliferation, migration, and invasion of cervical cancer cell and is upregulated by human papillomavirus 16 E7 oncoprotein. Gynecol Obstet Inves. ;:  WB ;  Human.  
保存条件 Store at -20℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 简介
蛋白质定量是蛋白质研究的基础工作之一。BCA (bicinchoninic acid)是理想的蛋白质定量方法;是当前比Lowry法更优越的专用于检测总蛋白质含量的产品。该方法以快速灵敏、稳定可靠,对不同种类蛋白质检测的变异系数非常小而倍受专业人士的青睐。BCA法测定蛋白浓度不受绝大部分样品中的化学物质的影响。在组织细胞裂解实验中,常用浓度的去垢剂SDS,Triton X-100,Tween不影响检测结果,但受螯合剂(EDTA,EGTA)、还原剂(DTT,巯基乙醇)和脂类的影响。实验中,若发现样品稀释液或裂解液本身背景值较高,可试用Bradford蛋白浓度测定试剂盒。该产品不受此影响,因此与之配合使用,可免除您实验中的许多烦恼。
基本原理:碱性条件下,蛋白将Cu2+还原为Cu+, Cu+与BCA试剂形成紫颜色的络合物,测定其在562nm处的吸收值,并与标准曲线对比,即可计算待测蛋白的浓度。
BCA试剂的蛋白质测定范围是10-2000ug/ml

使用说明:
1. 配制工作液: 根据标准品和样品数量,按体积比A :B (50:1)配制适量BCA工作液,充分混匀。BCA工作液室温24小时内稳定。
2. 稀释标准品:取10微升标准品用PBS稀释至100ul(标准品一般可用PBS稀释),使终浓度为0.5mg/ml。将标准品按0, 1, 2, 4, 8, 12, 16, 20ul加到96孔板的蛋白标准品孔中,加PBS补足到20ul。
3. 加适当体积样品到96孔板的样品孔中,补加PBS到20ul。
4. 各孔加入200ul BCA工作液,37℃放置30分钟。
5. 冷却到室温,用酶标仪562nm测定OD值,根据标准曲线计算出蛋白浓度。

注意事项:
1. 在低温条件或长期保存出现沉淀时,可搅拌或37℃温育使溶解, 如发现细菌污染则应丢弃。
2. 样品中若含有EDTA、EGTA、DTT、硫酸铵、脂类会影响检测结果,请试用Bradford蛋白浓度测定试剂盒;高浓度的去垢剂也影响实验结果,可用TCA沉淀去除干扰物质。
3.要得到更为精确的蛋白浓度结果,每个蛋白梯度和样品均需做复孔, 每次均应做标准曲线。
4.当试剂A和B混合时可能会有浑浊,但混匀后就会消失,工作液在密闭情况下可保存1周。
5.需准备37℃水浴或温箱、酶标仪或普通分光光度计,测定波长为540-595nm之间,562nm最佳。酶标仪需与96孔酶标板配套使用。使用分光光度计测定蛋白浓度时,试剂盒测定的样品数量会因此而减 少。使用温箱孵育时,应注意防止因水粉蒸发影响检测结果。


本产品自2018年4月1日起配方升级,规格和包装有所调整,请以官网信息为准。
产品图片
C05-02001
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