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BiossECL Plus WB Substrate (C05-07004)  
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说明书: 下载
12.5ml×2/280.00元
50ml×2/880.00元
250ml×2/3200.00元
大包装/询价
产品编号 C05-07004
英文名称 BiossECL Plus WB Substrate
中文名称 BiossECL超敏发光液
别    名 ECL Western Blotting Substrate; BiossECL Plus Western Blotting Substrate Kit; ECL Western Blotting Substrate Kit; Ultrasensitive ECL Kit; BiossECL Plus; Western Blotting Luminol Reagent;   极超敏ECL化学发光试剂盒; UltraSignal 超敏ECL化学发光底物;UltraSignal 超敏ECL化学发光液; ECL Plus超敏发光液; ECL超敏发光液; ECL超敏发光底物; ECL发光液; ECL Plus级发光液; 超敏化学发光检测试剂盒; Western萤光检测试剂盒; 鲁米诺化学发光试剂; 增强型ECL 化学发光检测试剂盒(即用型);
Specific References  (16)     |     C05-07004 has been referenced in 16 publications.
[IF=17.694] Guo, Yuxin. et al. In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses. NAT COMMUN. 2022 Nov;13(1):1-20  
[IF=10.317] Zhuo Wang. et al. Targeted galectin-7 inhibition with ultrasound microbubble targeted gene therapy as a sole therapy to prevent acute rejection following heart transplantation in a Rodent model. Biomaterials. 2020 Dec;263:120366  
[IF=6.792] Li Z et al. Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats. Environ Pollut . 2020 Oct;265(Pt A):115025.  WB ;  
[IF=4.872] Junyu Hou. et al. Polystyrene microplastics lead to pyroptosis and apoptosis of ovarian granulosa cells via NLRP3/Caspase-1 signaling pathway in rats. Ecotox Environ Safe. 2021 Apr;212:112012  other ;  
[IF=3.848] Zuo Qiang. et al. Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway. Acta Bioch Bioph Sin. 2021 Sep;:  WB ;  human .  
[IF=3.654] Mengying Zhang. et al. Preparation of Black pepper (Piper nigrum L.) essential oil nanoparticles and its antitumor activity on triple negative breast cancer in vitro. J FOOD BIOCHEM. 2022 Sep;:e14406  Other ;  Other.  
[IF=3.647] Zhang C et al. Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in triple negative breast cancer cellsLife Sci.2020 Dec 10;266:118886.  WB ;  
[IF=3.34] Zhang, Yi-Dan. et al. Role of synaptotagmin 13 (SYT13) in promoting breast cancer and signaling pathways. CLIN TRANSL ONCOL. 2023 Jan;:1-12  
[IF=3.298] Zhongxiao Zhou. et al. Formononetin regulates endothelial nitric oxide synthase to protect vascular endothelium in deep vein thrombosis rats:. INT J IMMUNOPATH PH. 2022;():  
[IF=3.056] Shengda Li. et al. Polystyrene microplastics induce blood–testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats. 2021 Apr 25  Other ;  
[IF=2.952] Guanglei Han. et al. Salvianolic acid B acts against non‑small cell lung cancer A549 cells via inactivation of the MAPK and Smad2/3 signaling pathways. Mol Med Rep. 2022 May;25(5):1-11  Other ;  Other.  
[IF=2.903] Fangming Guo. et al. Shenfu Administration Improves Cardiac Fibrosis in Rats With Myocardial Ischemia-Reperfusion Through Adenosine A2a Receptor Activation:. Hum Exp Toxicol. 2022;():  Other ;  Other.  
[IF=2.745] Zhonghua An. et al. Chamomile Essential Oil: chemical constituents and antitumor activity in MDA-MB-231 cells through PI3K/Akt/mTOR signaling pathway. CHEM BIODIVERS. 2023 Mar;:e202200523  
[IF=2.65] Sitong Ming. et al. Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA. EVID-BASED COMPL ALT. 2022 Sep 07;2022:3188292  Other ;  Other.  
[IF=2.315] Lin Chen. et al. Preparation, structural characterization and neuroprotective effects of polysaccharides from the pericarp of Zanthoxylum bungeanum Maxim against H2O2-induced oxidative damage in PC12 cells. S Afr J Bot. 2021 Nov;142:165  WB ;  Rat.  
[IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
保存条件 Store at 2-8℃. Protect from light for one year.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 简介
BiossECL超敏发光液直接或间接检测与辣根过氧化物酶HRP关联的蛋白或核酸底物。这一独特的发光底物系统是目前最灵敏的商业化荧光ECL检测试剂,具有极高灵敏度和高信噪比,可检测出10-100 fg的微量抗原;发光迅速,荧光可使X 感光胶片感光达12小时以上,特别适用于痕量蛋白或核酸检测。同时该试剂允许使用更高的抗体稀释倍数(1:2000-1:10000),极其节省抗体。

试剂盒内容
25ml规格:A液12.5ml + B液12.5ml
100ml规格:A液50ml + B液50ml
500ml规格:A液250ml + B液250ml

显色步骤
1、执行常规SDS-PAGE、转膜和Western Blot封闭、一抗孵育、二抗孵育操作。
2、二抗适用HRP标记二抗,适用恰当的稀释比例。
3、制备ECL工作液:A液和B液等体积的混合,例如1ml A液和1ml B液混合,混匀后即可使用。用量以充分覆盖印迹膜为基准,每10 cm2膜需要大约1 ml工作液。
4、将印迹膜有蛋白的一面朝上平整的铺在一张平板上,加上配好的发光底物工作液。
5、将工作液均匀滴加在印迹膜上,孵育1-5min。可将一洁净的保鲜膜或透明的玻璃纸平整的铺在孵育有工作液的印迹膜上,轻轻赶出其间的气泡。
6、在暗室中取一张X胶片小心置于膜上,曝光5秒至1分钟,立即显影定影。
7、根据其发光的强度,缩短或延长X片的曝光时间(对微弱信号,曝光时间可延长至数小时),或者曝光一系列不同的时间后再显影定影挑选一张满意的。也可用合适的照相器材直接记录蛋白膜的化学发光图像。

安全性:
无特殊毒性,按普通化学品处理。如果不慎与眼、皮肤和衣物接触,请立刻用大量清水冲洗。

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