| 产品编号 | D-9604 |
| 英文名称 | Cy5,SE |
| 中文名称 | 荧光标记染料Cy5 |
| 别 名 | 荧光染料; 荧光素Cy5 |
| 保存条件 | Store at -20 °C for one year. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC). Due to inherently high extinction coefficient, this dye is also easily detected by naked eye on gels, and in solution. Cy5 become very popular to replace far red fluorescent dyes, because of its high extinction coefficient (as small as 1 nanomol can be detected in gel electrophoresis by naked eye) and of its fluorophore emission maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. The scanners actually use different laser emission wavelengths (typically 532 nm and 635 nm) and filter wavelengths (550-600 nm and 655-695 nm) to avoid background contamination. They are thus able to easily distinguish colors from Cy3 and from Cy5, and also able to quantify the amount of Cy3 and Cy5 labeling in one sample (multiparametric detection). 菁染料是性能优良的荧光标记染料，摩尔吸光系数在荧光染料中是最高的，其琥珀酰亚胺酯是最常用的脂肪氨基标记试剂，广泛用于蛋白、抗体、核酸及其他生物分子的标记和检测。通过改变次甲基链的长度, 可改变其荧光发射波长，每增加一个双键，按照Huoffman 规则正好红移约100nm。 菁染料Cy3 和Cy5 已成为基因芯片的首选荧光标记物; 另外,Cy5, Cy5.5 和Cy7 的吸收在近红外区背景非常低，是荧光强度最高、最稳定的长波长染料。特别适合于活体小动物体内成像代替放射性元素。 花菁染料Cy5, SE是一种为数不多的近红外荧光标记染料，它产生的红色荧光背景低、荧光强度高，而且荧光稳定性好。Cy5, SE常被应用于生物分子标记，荧光成像及其他荧光生物分析，例如标记多肽、蛋白质和核酸等。Cy5, SE 荧光标记染料与蛋白质结合后可以显著增强荧光强度。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
