| 产品编号 | bs-23361R |
| 英文名称 | GCLM |
| 中文名称 | γ 谷氨酰半胱氨酸连接酶调节亚基抗体 |
| 别 名 | Glutamate-Cysteine Ligase Modifier Subunit; Gamma-Glutamylcysteine Synthetase Regulatory Subunit; Glutamate--Cysteine Ligase Modifier Subunit; Gamma-ECS Regulatory Subunit; GCS Light Chain; GLCLR; Glutamate-Cysteine Ligase (Gamma-Glutamylcysteine Synthetase), Regulatory (30.8kD); Glutamate-Cysteine Ligase Modifier Subunit Delta2 Alternative Splicing; Glutamate--Cysteine Ligase Regulatory Subunit; Glutamate-Cysteine Ligase Regulatory Protein; Glutamate-Cysteine Ligase, Modifier Subunit; Gamma-Glutamylcysteine Synthetase; GSC Light Chain; GSH0_HUMAN; |
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Specific References (2) | bs-23361R has been referenced in 2 publications.
[IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268. WB ; Human.
[IF=4.253] Mengru Li. et al. The leaves of Scutellaria baicalensis Georgi attenuate brain aging in D-galactose-induced rats via regulating glutamate metabolism and Nrf2 signaling pathway. EXP GERONTOL. 2022 Dec;170:111978 WB ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 转录调节因子 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCLM: 31-120/274 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] Function: This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate. Subunit: Heterodimer of a catalytic heavy chain and a regulatory light chain. Subcellular Location: Cytosol Tissue Specificity: In all tissues examined. Highest levels in skeletal muscle. Similarity: Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily. SWISS: P48507 Gene ID: 2730 Database links: Entrez Gene: 2730 Human Entrez Gene: 14630 Mouse Omim: 601176 Human SwissProt: P48507 Human SwissProt: O09172 Mouse Unigene: 315562 Human Unigene: 292676 Mouse Unigene: 2460 Rat |
| 产品图片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-GCLM (bs-23361R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD 
Sample:
Hela(Human) Cell Lysate at 30 ug A673(Human) Cell Lysate at 30 ug Primary: Anti-GCLM (bs-23361R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD 
Sample:
Muscle (Mouse) Lysate at 40 ug Primary: Anti-GCLM (bs-23361R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD 
Sample:
Kidney (Mouse) Lysate at 40 ug Primary: Anti-GCLM (bs-23361R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
