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Rabbit Anti-B7-1/CD80  antibody (bs-23296R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-23296R
英文名称 B7-1/CD80
中文名称 刺激分子B7-1蛋白抗体
别    名 Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_MOUSE; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80.  
研究领域 肿瘤  免疫学  干细胞  b-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse,Rat
产品应用 WB=1:500-1000, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 32kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse B7-1/CD80: 1-100/306 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

Function:
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Subcellular Location:
Membrane.

Tissue Specificity:
Expressed on activated B-cells, macrophages and dendritic cells.

Similarity:
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q00609

Gene ID:
12519

Database links:

Entrez Gene: 941 Human

Entrez Gene: 12519 Mouse

Omim: 112203 Human

SwissProt: P33681 Human

SwissProt: Q00609 Mouse

Unigene: 838 Human

Unigene: 89474 Mouse



产品图片
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD80 (bs-23296R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 60 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-CD80 (bs-23296R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 60 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
SP2/0 (Mouse) CellLysate at 30 ug
Primary: Anti-B7-1/CD80 (bs-23296R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 60 kD
Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 3: SP2/0 (Mouse) Cell Lysate at 30 ug
Lane 4: Thymus (Rat) Lysate at 40 ug
Lane 5: Raji (Human) Cell Lysate at 30 ug
Lane 6: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-B7-1/CD80 (bs-23296R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 60 kD
Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Thymus (Mouse) Lysate at 40 ug
Lane 4: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 5: Sp2/0 (Mouse) Cell Lysate at 30 ug
Primary: Anti-B7-1/CD80 (bs-23296R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 62 kD
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