| 产品编号 | bsm-33019M |
| 英文名称 | GFP |
| 中文名称 | 绿色荧光蛋白单克隆抗体 |
| 别 名 | Green fluorescence protein; 绿色荧光蛋白 GFP; 绿色荧光蛋白 (GFP); |
| 产品类型 | 标签抗体 |
| 研究领域 | 细胞生物 海洋生物 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 4G6 |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:50000-500000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant Green Fluorescence Protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Subunit: Monomer. Tissue Specificity: Photocytes. Post-translational modifications: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Similarity: Belongs to the GFP family. SWISS: P42212 Gene ID: 107331116 Database links:
|
| 产品图片 |
Sample:
GFP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug Primary: Anti-GFP-Tag (bsm-33019M) at 1/1000000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 30 kD 
Transformed (GFP or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bsm-33019M (Anti-GFP) at dilution of 1:1,000,000 incubated at 4℃ overnight.
Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug Primary: Anti-GST tag (bsm-33019M) at 1/5000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD 
Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 2 ug Primary: Anti-GST tag (bsm-33019M) at 1/5000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD 
Sample:
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug Primary: Anti-GFP-Tag (bsm-33019M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
