产品编号 | bsm-33318M |
英文名称 | Mouse Anti-Firefly Luciferase antibody |
中文名称 | 荧光素酶单克隆抗体 |
别 名 | ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase. |
Specific References (1) | bsm-33318M has been referenced in 1 publications.
[IF=6.58] Jiang Chen. et al. Inhibition of SARS-CoV-2 pseudovirus invasion by ACE2 protecting and Spike neutralizing peptides: An alternative approach to COVID19 prevention and therapy. Int J Biol Sci. 2021; 17(11): 2957–2969 IF,IHC ; Human.
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产品类型 | 标签抗体 |
研究领域 | 昆虫 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 11D6 |
交叉反应 | Recombinant protein |
产品应用 | WB=1:500-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 61kDa |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Luciferase comes from the North American firefly (Firefly) |
亚 型 | IgG1 |
纯化方法 | affinity purified by Protein G |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. Function: Produces green light with a wavelength of 562 nm. Subcellular Location: Peroxisome. Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. SWISS: P08659 Gene ID: N/A Database links: SwissProt: P08659 Firefly (Photinus pyralis) |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |