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Mouse Anti-eNOS  antibody (bsm-33176M)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bsm-33176M
英文名称 eNOS
中文名称 一氧化氮合成酶-3(内皮型)单克隆抗体
别    名 NOS-3; cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.  
Specific References  (1)     |     bsm-33176M has been referenced in 1 publications.
[IF=4.456] Ecem Kaya-Sezginer. et al. Sodium butyrate ameliorates erectile dysfunction through fibrosis in a rat model of partial bladder outlet obstruction. ANDROLOGY-US. 2022 Jul;:  WB, IHC ;  Rat.  
研究领域 细胞生物  神经生物学  信号转导  合成与降解  内皮细胞  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 8G1
交叉反应 Human,Mouse,Rat
产品应用 WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 133kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human eNos 
亚    型 IgG
纯化方法 affinity purified by Protein G
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

Function:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Subunit:
Homodimer. Interacts with NOSIP and NOSTRIN.

Subcellular Location:
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

Tissue Specificity:
Platelets, placenta, liver and kidney.

Post-translational modifications:
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.

Similarity:
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.

SWISS:
P70313

Gene ID:
18127

Database links:

Entrez Gene: 287024 Cow

Entrez Gene: 4846 Human

Entrez Gene: 18127 Mouse

Entrez Gene: 24600 Rat

Omim: 163729 Human

SwissProt: P29473 Cow

SwissProt: P29474 Human

SwissProt: P70313 Mouse

SwissProt: Q62600 Rat

Unigene: 647092 Human

Unigene: 258415 Mouse

Unigene: 44265 Rat



合成与降解(Synthesis and Degradation)
催化生物体内一氧化氮(NO)生成的酶。分神经型一氧化氮合成的酶(nNOS or NOS-1)、诱导型一氧化氮合成的酶(iNOS or NOS-2)、内皮型一氧化氮合成的酶(eNOS or NOS-3)。此抗体识别分子量为135kDa的内皮型一氧化氮合成的酶。
产品图片
Sample: Liver(mouse) Lysate at 40 ug
Primary: Anti-eNOS (bsm-33176M)at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 133kD
Observed band size: 135kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Placenta (Mouse) Lysate at 40 ug
Primary: Anti-eNOS (bsm-33176M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 135 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (eNOS/NOS-3) Monoclonal Antibody, Unconjugated (bsm-33176M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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